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elisa human proprotein convertase 9 pcsk9 quantikine elisa kit  (R&D Systems)


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    R&D Systems elisa human proprotein convertase 9 pcsk9 quantikine elisa kit
    Elisa Human Proprotein Convertase 9 Pcsk9 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+elisa+kits/Human+Proprotein+Convertase+9%2FPCSK9+Quantikine+ELISA+Kit/pmc13091390-93-23-31
    Average 95 stars, based on 93 article reviews
    elisa human proprotein convertase 9 pcsk9 quantikine elisa kit - by Bioz Stars, 2026-09
    95/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Effects of locally applied water-filtered infrared a irradiation adjunctive to iloprost and carbon dioxide hand baths in patients with systemic sclerosis and severe Raynaud's phenomenon - a randomized controlled trial.
    Article Snippet: .. IL-6 and VEGF concentrations were quantified using Quantikine® ELISA kits (R&D Systems) according to the manufacturer’s instructions. .. Absorbance was measured at 450 nm (reference 570 nm) on a SUNRISE Reader (Tecan, Austria).

    Article Title: Klebsiella pneumoniae infection induces an S100A8/A9-mediated autocrine loop in human airway epithelium to amplify inflammation
    Article Snippet: .. The concentrations of secreted S100A8/A9 heterodimer, IL-8, and IL-6 were quantified by commercial Quantikine ELISA kits (R&D Systems, Cat# DS8900 for S100A8/A9, D8000C for IL-8, and D6050B for IL-6). .. Following the kit instructions, absorbance was measured (F50, TECAN).

    Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration.
    Article Snippet: .. AR TI CL E IN P RE SS ELISA for IFN- and IL-17A quantification: Secreted levels of human interferon-beta (IFN-) and interleukin-17 (IL-17) in the iPSC-RPE conditioned media were measured using Quantikine ELISA kits (R&D Systems, Cat. #DIFNB0 for IFN-; Cat. #D1700 for IL-17A) according to the manufacturer's instructions. ..

    Article Title: SPP1 high fibrogenic macrophages mediate protective fibrotic remodeling and promote vascular stability in hypertension-associated aortic dissection
    Article Snippet: .. Cytokines (IL-1β, IL-6, TNF-α, TGF-β1/β2, OPN) were quantified using Quantikine ELISA kits (R&D Systems) following the manufacturer’s instructions and reported as absolute concentrations or fold-changes. .. Bone marrow cells were isolated from the femurs and tibias of 6-week-old C57BL/6 J mice and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin (Thermo Fisher Scientific), and 25 ng/mL recombinant murine M-CSF (rM-CSF) for 7 days to induce macrophage differentiation.

    Article Title: Potential biomarkers and preventative interventions for muscle wasting in adolescent and young adults with cancer: A translational study.
    Article Snippet: IL-6 levels in mouse and patient samples The Multi-Analyte ELISArray Kit (Qiagen) was used to quantify the IL-6 levels in the gastrocnemius muscle and plasma samples from 10 different mice from each group. .. Validation and quantification of IL-6 were performed using Quantikine ELISA Kits (R&D Systems, Minneapolis, MN). ..

    Article Title: Triggering Receptor Expressed on Myeloid Cells-2 Regulates Innate Lymphoid Cell Levels in Bleomycin-Induced Pulmonary Fibrosis.
    Article Snippet: .. Tissue- protective amphiregulin (AREG) levels in lung tissue were analyzed using Quantikine ELISA kits (M6000B, R&D Systems Inc., Minneapolis, MN, USA). .. Mouse lung tissues were homogenized in lysis buffer containing protease inhibitors (1862209, Thermo, Waltham, MA, USA).

    Article Title: Secretory VSMC-derived THBS1 promotes macrophage M1 polarization and inflammation in intracranial aneurysms via the CD47/NF-κB axis
    Article Snippet: .. At the protein secretion level, commercially available Quantikine ® ELISA kits (R&D Systems, DY201 for IL-1β; DY210 for TNF-α) were used to quantify IL-1β and TNF-α concentrations in the lower-chamber supernatants following the manufacturer’s instructions. ..

    other:


    Biomarker Discovery:

    Article Title: Potential biomarkers and preventative interventions for muscle wasting in adolescent and young adults with cancer: A translational study.
    Article Snippet: IL-6 levels in mouse and patient samples The Multi-Analyte ELISArray Kit (Qiagen) was used to quantify the IL-6 levels in the gastrocnemius muscle and plasma samples from 10 different mice from each group. .. Validation and quantification of IL-6 were performed using Quantikine ELISA Kits (R&D Systems, Minneapolis, MN). ..



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    Image Search Results


    circSMAD4 drives tumor-educated M2-like polarization of macrophages and promotes tumor-cell aggressiveness. (A) Workflow for generating TC-hMDMs and TC-BMDMs, circSMAD4 knockdown, and downstream functional assays. (B) RT–qPCR analysis of M1-associated markers (MHC-II [HLA-DRA in TC-hMDMs; H2-Ab1 in TC-BMDMs], NOS2, and CD86) and M2-associated markers (CD163, CD206, and ARG1) in TC-hMDMs and TC-BMDMs. (C) Representative flow-cytometry histograms for HLA-DR, iNOS, CD86, CD163, CD206, and ARG1 in TC-hMDMs. Gating strategy and marker thresholds were defined based on FMO controls (see ). (D) Flow-cytometry quantification of marker-positive cells in TC-hMDMs and TC-BMDMs. (E) ELISA of IL-10, TGF-β, and iNOS in culture supernatants. (F) CCK-8 assays of A549 and LLC cells. (G) Colony-formation assays of A549 and LLC cells with quantification. (H) Bioluminescence-based growth readouts of patient-derived LUAD organoids (PDO #1 and PDO #2) after co-culture with TC-hMDMs. (I) Immunoblot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin) in A549 and LLC cells. (J) Transwell migration and invasion assays of A549 and LLC cells with quantification. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.

    Journal: Non-coding RNA Research

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma

    doi: 10.1016/j.ncrna.2026.03.003

    Figure Lengend Snippet: circSMAD4 drives tumor-educated M2-like polarization of macrophages and promotes tumor-cell aggressiveness. (A) Workflow for generating TC-hMDMs and TC-BMDMs, circSMAD4 knockdown, and downstream functional assays. (B) RT–qPCR analysis of M1-associated markers (MHC-II [HLA-DRA in TC-hMDMs; H2-Ab1 in TC-BMDMs], NOS2, and CD86) and M2-associated markers (CD163, CD206, and ARG1) in TC-hMDMs and TC-BMDMs. (C) Representative flow-cytometry histograms for HLA-DR, iNOS, CD86, CD163, CD206, and ARG1 in TC-hMDMs. Gating strategy and marker thresholds were defined based on FMO controls (see ). (D) Flow-cytometry quantification of marker-positive cells in TC-hMDMs and TC-BMDMs. (E) ELISA of IL-10, TGF-β, and iNOS in culture supernatants. (F) CCK-8 assays of A549 and LLC cells. (G) Colony-formation assays of A549 and LLC cells with quantification. (H) Bioluminescence-based growth readouts of patient-derived LUAD organoids (PDO #1 and PDO #2) after co-culture with TC-hMDMs. (I) Immunoblot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin) in A549 and LLC cells. (J) Transwell migration and invasion assays of A549 and LLC cells with quantification. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.

    Article Snippet: For mouse experiments, mouse IL-10 was measured using the Mouse IL-10 ELISA Kit (R&D Systems, Cat# M1000B), and mouse TGF-β1 was measured using the Mouse TGF beta-1 ELISA Kit (Invitrogen, Cat# BMS608-4), following the manufacturers’ instructions.

    Techniques: Knockdown, Functional Assay, Quantitative RT-PCR, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Derivative Assay, Co-Culture Assay, Western Blot, Migration